Camera Web Saos Corporation Euthanasia

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Deep-red fluorescent molecular probes are described that have dendritic molecular architecture with a squaraine rotaxane core scaffold and multiple peripheral iminodiacetate groups as the bone targeting units. Iminodiacetates have inherently lower bone affinity than bisphosphonates and a major goal of the study was to determine how many appended iminodiacetate groups are required for effective deep-red fluorescence imaging of bone in living rodents. A series of in vitro and in vivo imaging studies showed that a tetra(iminodiacetate) probe stains bones much more strongly than an analogous bis(iminodiacetate) probe. In addition, a control tetra(iminodiapropionate) probe exhibited no bone targeting ability.

The tetra(iminodiacetate) probe targeted the same regions of high bone turnover as the near-infrared bisphosphonate probe OsteoSense®750. Longitudinal studies showed that the fluorescence image signal from living mice treated with the tetra(iminodiacetate) probe was much more stable over 19 days than the signal from OsteoSense®750. The narrow emission band of the tetra(iminodiacetate) probe makes it very attractive for inclusion in multiplex imaging protocols that employ a mixture of multiple fluorescent probes in preclinical studies of bone growth or in fluorescence guided surgery.

Dec 1, 2013 - Mice that received Evans blue were euthanized by cardiac perfusion. SAOS-2 cells were maintained in Dulbecco's Modified Eagle's Media. To BMPR-II (BD Biosciences, 1:100) and actin (Santa Cruz Biotechnology, Inc; 1:2,000). Tumor sections were captured using the iSight camera on an iPhone 4S. Higher standard deviations were detected for the Raman band at 780–800 cm −1 in both apoptotic and necrotic Saos-2 cells. In addition, all treated Saos-2 cells showed a diminishing peak at 1052 cm −1 ( Supplemental Figure S3 ).

The results also suggest that molecules or nanoparticles bearing multivalent iminodiacetate groups have promise as bone targeting agents with tunable properties for various pharmaceutical applications. Introduction The extracellular matrix of bone tissue has high mineral content, and is especially rich in Ca 2+ salts. A common feature of most bone-seeking agents is a strong affinity for metal cations. – For example, commercially available fluorescent dyes with Ca 2+ chelation ability have been used for several decades to stain samples of bone and teeth. – Typically, the dyes emit visible wavelengths, which is a limitation for in vivo studies due to poor penetration of visible light through skin and tissue. It is well recognized that biological imaging is improved by employing dyes that emit in the 650 – 900 nm window. Recently, several near-infrared bone probes have been reported and in most cases the molecules are cyanine dyes with appended bisphosphonates as the bone targeting groups.